A gold nanoparticle-based immunosensor for the chemiluminescence detection of the hepatitis B surface antigen
| Autor: | Soheila Sabouri, Mahdi Boutorabi, Mostafa Shourian, Hedayatollah Ghourchian |
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| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Anal. Methods. 6:5059-5066 |
| ISSN: | 1759-9679 1759-9660 |
| DOI: | 10.1039/c4ay00461b |
| Popis: | A sensitive immunoassay method was developed for the detection of HBsAg. A chemiluminescence label was prepared based on the co-immobilization of a secondary antibody and luminol on gold nanoparticles. HBsAg was targeted by a primary antibody, immobilized in polystyrene wells and a secondary antibody, co-immobilized on luminol–gold nanoparticles. After the formation of the immune sandwich, the luminescence intensity was recorded in the presence of hydrogen peroxide as an oxidant agent and Au3+ as an efficient catalyst for luminol oxidation. HBsAg was detected in the linear concentration range from 0.12 to 30 ng mL−1 and had the detection limit of 14 pg mL−1. The proposed method has been successfully applied to determine the HBsAg in patient serums. This method has excellent precision, a good detection limit, a wide linear range, a low cost and is user friendly. With these features, the proposed method is suitable for measuring antigen in patient serums. |
| Databáze: | OpenAIRE |
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