Autor: |
Daniela Pavingerová, M. Ondřej, Jaroslav Matoušek |
Rok vydání: |
1983 |
Předmět: |
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Zdroj: |
Zeitschrift für Pflanzenphysiologie. 112:427-433 |
ISSN: |
0044-328X |
DOI: |
10.1016/s0044-328x(83)80148-2 |
Popis: |
Summary Seeds of Arabidopsis thaliana plants, differentiated from nopaline and octopine crown galls, were harvested and sown on agar medium in bulk. Approximately 5% of the seedlings of both types showed an abnormal phenotype (dwarf type of growth and/or curled leaves). When their calli were subcultured on medium without growth regulators and cultivated in the light, 47 clones expressed opine synthesizing activities which became detectable after 2–5 subcultivations. Seed progeny of a single nopaline teratoma was tested separately. The differentiated floral axes were completly or partly sterile due to the alternations in floral development. In F 1 and F 2 generations, NpDH activity was found in some of the plants; undifferentiated calli appeared occasionally on roots and stems. These calli showed NpDH activity and they prove the transmission of tumor markers to the F 2 generation. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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