In vitro selection of aptamers that recognize a monosaccharide

Autor:	Yasuharu Kawase, Junji Kawakami, Naoki Sugimoto
Rok vydání:	1998
Předmět:	chemistry.chemical_classification Hydrogen bond Stereochemistry Aptamer Mannose RNA Biochemistry Small molecule Analytical Chemistry chemistry.chemical_compound chemistry Galactose Nucleic acid Environmental Chemistry Monosaccharide Spectroscopy
Zdroj:	Analytica Chimica Acta. 365:95-100
ISSN:	0003-2670
DOI:	10.1016/s0003-2670(97)00579-5
Popis:	Attempts, were made to obtain RNAs that can bind to monosaccharides (galactose, glucose, and mannose) by in vitro selection. As a result, an RNA library that can bind to galactose with outstanding affinity has been obtained. Furthermore, the library was able to distinguish galactose from other monosaccharides, though they have closely similar structures. After another selection in order to obtain fast binders, a selected library tightly bound to galactose was obtained but it lost selectivity to galactose. This selectivity seemed to be derived from hydrogen bond geometry with the epimeric hydroxyl groups of monosaccharides. The results show an ability of RNAs to recognize any small molecule which consists of only carbon, hydrogen and oxygen.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::57cd2f1311e456e1ba2243e84ebe1815 https://doi.org/10.1016/s0003-2670(97)00579-5 Zobrazit plný text záznamu Full Text from ScienceDirect