Matrix effects and selectivity issues in LC-MS-MS

Autor:	D. Watson, J. Smeraglia, S. F. Baldrey
Rok vydání:	2002
Předmět:	Bioanalysis Electrospray Reproducibility Chromatography Tandem Chemistry Organic Chemistry Clinical Biochemistry Analytical chemistry Biochemistry High-performance liquid chromatography Analytical Chemistry Sample preparation Solid phase extraction Selectivity
Zdroj:	Chromatographia. 55:S95-S99
ISSN:	1612-1112 0009-5893
DOI:	10.1007/bf02493363
Popis:	Matrix effect is the effect on an analytical method caused by all other components of the sample except the specific compound to be quantified. Matrix effects and selectivity issues have long been associated with bioanalytical techniques. However the high incidence of matrix effects in liquid chromatographic tandem mass spectrometric (LC-MS-MS) methods has led to a greater understanding of the factors which contribute to these effects. A number of approaches have been investigated to improve reproducibility and robustness of LC-MS-MS methods that are subjected to matrix effect. The modifications described herein relate to instrumentation and methodological issues and include modified ionisation, ionisation switching, extraction modification and gradient high pressure liquid chromatography (HPLC) techniques and have demonstrated significantly improved robustness of complex bioanalytical methods to avoid matrix-related issues.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::57c9eba16317cf08ad3665385e2ace6f https://doi.org/10.1007/bf02493363 Zobrazit plný text záznamu Full text from SpringerLink