Xanthoangelol and 4-hydroxyderrcin suppress obesity-induced inflammatory responses

Autor: Haruya Takahashi, Teruo Kawada, Ryuma Ikutani, Shigeru Murakami, Masahiko Taniguchi, Huei-Fen Jheng, Yongjia Li, Nobuyuki Takahashi, Shan Lin, Rina Yu, Tsuyoshi Goto, Kimiye Baba
Rok vydání: 2016
Předmět:
Zdroj: Obesity. 24:2351-2360
ISSN: 1930-7381
DOI: 10.1002/oby.21611
Popis: Objective Obesity-induced inflammation plays a pivotal role in the pathogenesis of insulin resistance and type 2 diabetes. Xanthoangelol (XA) and 4-hydroxyderrcin (4-HD), phytochemicals extracted from Angelica keiskei, have been reported to possess various biological properties. Whether XA and 4-HD alleviate obesity-induced inflammation and inflammation-induced adipocyte dysfunction was investigated. Methods For the in vitro study, a co-culture system composed of macrophages and adipocytes and macrophages stimulated with conditioned medium derived from fully differentiated adipocytes was conducted. For the in vivo study, mice were fed a high-fat diet supplemented with XA for 14 weeks. Results XA and 4-HD suppressed inflammatory factors in co-culture system. Moreover, treatment of RAW macrophages with XA and 4-HD moderated the suppression of uncoupling protein 1 promoter activity and gene expression in C3H10T1/2 adipocytes, which was induced by conditioned medium derived from LPS-stimulated RAW macrophages. Also, XA and 4-HD inhibited c-Jun N-terminal kinase phosphorylation, nuclear factor-κB, and activator protein 1, the last two being transcription activators in activated macrophages. Furthermore, in mice fed the high-fat diet, XA reduced inflammatory factors within the white adipose tissue. Conclusions These results suggest that XA and 4-HD might be promising phytochemicals to suppress obesity-induced inflammation and inflammation-induced adipocyte dysfunction.
Databáze: OpenAIRE
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