| Popis: |
In this paper, we propose a software application for validating the automated decellularization process for a rat heart. This pilot study implemented a latex balloon that simulates a heart in which initially we introduced dye, using a syringe pump for clinical use. Then we pumped water with a controlled flow, so finally, we replaced this dye with injected water. With this design, we can validate the software application because it automatically detects the moment when the simulated heart completely discolored, and turned off the entire system, which is equivalent to total real heart decellularization. During this process, photos were automatically taken at a certain interval, selected by the user. Photos were processed according to the selected area at the beginning of each experiment. The data collected was stored and the evolution of the dye from the simulated heart was plotted. In other words, once validated, this application can be used further in the entire system created specifically for the decellularization of a rat heart. Because decellularization is a generally long process, which currently requires a lot of manual operations and supervision, the main goal in our study is to design an automated system for the entire decellularization process, so that it eliminates collecting samples for the method of ultraviolet spectrophotometry. |
