Preparation of 99mTc-isosulfan blue for lymph node localization in rats 99mTc-isosulfan blue for lymph node localization
| Autor: | Baha Zengel, Makbule Asikoglu, Gökhan Ertan, Ulkem Yararbas, Derya Ilem-Ozdemir |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Pathology
medicine.medical_specialty Radiation business.industry Melanoma Sentinel lymph node Isosulfan Blue Sentinel node 010403 inorganic & nuclear chemistry medicine.disease 01 natural sciences 0104 chemical sciences 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Lymphatic system 030220 oncology & carcinogenesis medicine Lymph business Nuclear medicine Lymph node Gamma probe |
| Zdroj: | Applied Radiation and Isotopes. 118:338-342 |
| ISSN: | 0969-8043 |
| DOI: | 10.1016/j.apradiso.2016.10.007 |
| Popis: | The sentinel lymph node (SLN) is defined as the first regional lymph node to receive lymphatic drainage from a malignant tumor. Therefore, this node is a “sentinel” for second metastatic lymph node stations and for labeling regional tumor spread. For SLN detection, many surgeons preferred a combination of a preoperative injection of radiolabeled colloid and the intraoperative injection of blue dye. Under this combination protocol, nodes are considered to be “sentinel nodes” if they are radioactive and blue. The aim of this study is to develop a new single agent that combines both detection methods. For this purpose Isosulfan Blue (ISB) was radiolabeled by 99mTc with high labeling yield and stability. In vivo gamma scintigraphy studies were performed with rats. According to the scintigraphic studies, 99mTc-ISB shows rapid and high accumulation in both axillary (ALN) and popliteal lymph node (PLN). After the imaging study, extremity was opened and nodes were scanned for the radioactivity. According to performed study the lymph nodes were clearly seen to become blue and carried compound was sufficient to allow identification with a gamma probe. In conclusion, 99mTc-ISB has the potential to facilitate lymphatic mapping and subsequent sentinel node biopsy for solid malignancies such as breast cancer and melanoma. |
| Databáze: | OpenAIRE |
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