| Popis: |
Studies on the genotypic structure of the Altai white down breed by BLG, IGF-1, and CSN3 genes are presented. Genomic DNA was isolated from the blood of 113 animals using the Amplie Prime DNA-Sorb-B clinical extraction kit. Molecular genetic reactions were performed on a C1000 BioRad amplifier. Visualization and identification of the results were determined by flatbed electrophoresis in 2% agarose gel using the E-BoxCX5.TS-20.M gel-documentation system (France) in transmitted ultraviolet light. As a result, the frequency of genotypes and alleles was established as follows: BLGS1S1 – 17,7%, BLGS1S2 – 57,5, BLGS2S2 – 24, respectively; BLGS1 – 0,46; BLGS2 – 0,54; IGFAA – 2,7; IGFAB – 28,3 и IGFBB – 69,0%. The allele frequencies are IGFA – 0,17 and IGFB – 0,83. The CSN3 gene in Altai white down goats was monomorphic for the CSN3А allele. Analysis of the actual and theoretical distribution of BLG and IGF1 genotypes of goat genes indicates a genetic equilibrium in the flock. The down productivity of the studied animals is fleece - 680 g, down fineness -19.13 microns, and down length - 9.29 cm. Goats with the IGFAB genotype have more wool than the homozygotic IGFAA genotype. The fat content of the milk ranges from 6.03-6.24%, the protein content is 3.48-3.72%, and the lactose content is 4.56-4.90%. The study of milk quality indicators of Altai white down goats with different genotypes of BLG, IGF, and CSN3 revealed no significant differences. However, goats tend to increase milk fat and caloric content with the IGFAA genotype - 7.62. The studied goats of the Altai white down breed had a high equilibrium of indicators by live weight, length of down, down content in percentage, and down fineness - Cv˂10%. |
