An enthalpic approach to the analysis of the scanning force ligand rupture experiment

Autor:	Clive J. Roberts, Philip M. Williams, Saul J. B. Tendler, David E. Jackson, Adam Moore, Martyn C. Davies
Rok vydání:	1998
Předmět:	Molecular interactions Crystallography Chemistry Hydrogen bond Chemical physics Experimental data Ligand (biochemistry) Adiabatic process Combined approach Scanning Force Microscope
Zdroj:	Journal of the Chemical Society, Perkin Transactions 2. :253-258
ISSN:	1364-5471 0300-9580
DOI:	10.1039/a707165e
Popis:	The scanning force microscope has developed into a technique for the examination of key events involved in molecular interactions. Here we present a novel method to analyse the new family of force data obtained from this biophysical tool. Adiabatic mapping of the streptavidin–biotin interaction reveals a range of ligand rupture forces from 253 to 393 pN, which are in close agreement with experimental data. Analysis of the undocking process shows the importance of hydrogen bonding in this interaction. The potential of this combined approach as a method of studying rupture force data is highlighted.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::578b883a7d5a11d7f20493ac9534e7b7 https://doi.org/10.1039/a707165e Zobrazit plný text záznamu