Genome-wide identification and expression analysis of NRAMP transporter genes in Cucumis sativus and Citrullus lanatus
| Autor: | Cao Na, Li Guoquan, Zhu Fanghong, Zhang Huanxin, Yang Huidong |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Genetics 0303 health sciences Citrullus lanatus biology Cell Transporter Plant Science Horticulture biology.organism_classification 01 natural sciences Genome 03 medical and health sciences medicine.anatomical_structure Expression analysis medicine Identification (biology) Agronomy and Crop Science Gene Cucumis 030304 developmental biology 010606 plant biology & botany |
| Zdroj: | Canadian Journal of Plant Science. 101:377-392 |
| ISSN: | 1918-1833 0008-4220 |
| DOI: | 10.1139/cjps-2020-0062 |
| Popis: | Natural resistance-associated macrophage proteins (NRAMPs) are able to transport various metal ions across cell membranes, which play an important role in plant growth and development. Here, a survey of cucumber (Cucumis sativus L.) and watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] genomes found a total of five CsNRAMPs and four ClNRAMPs, respectively. Based on the phylogenetic relationships, CsNRAMPs and ClNRAMPs were clustered into three groups (I, II, and III). Five orthologous pairs were identified between cucumber and watermelon genome, and they were clustered on the same branch of the phylogenetic tree. The number of introns in CsNRAMPs and ClNRAMPs ranged from 3 to 13 and the genes from group I were more fragmented than those in group II. Subsequently, analysis of promoter sequences found that five putative transcription factors could act on NRAMPs. Moreover, CsNRAMPs and ClNRAMPs were differentially regulated by deficiencies of iron (Fe), manganese (Mn), copper (Cu), or zinc (Zn), along with toxicities of Fe, Mn, Cu, Zn, or cadmium (Cd). Functional analysis by heterologous expression in yeast indicated that CsNRAMP4 and ClNRAMP3 participate in Cd transport. Overall, the comprehensive analysis of CsNRAMPs and ClNRAMPs reported herein may pave the way for further investigations examining the regulation and functions of this gene family in cucumber and watermelon. |
| Databáze: | OpenAIRE |
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