Simvastatin Protects Human Melanocytes from H2O2-Induced Oxidative Stress by Activating Nrf2
Autor: | Xiuli Yi, Chunying Li, Zhe Jian, Weinan Guo, Yuanmin He, Kai Li, Lin Wang, Ling Liu, Gang Wang, Y. An, Tianwen Gao, Shuli Li, Q. Zhang, Yuqi Yang, Yuqian Chang, Tingting Cui, Pu Song, Weigang Zhang |
---|---|
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Dermatology Oxidative phosphorylation Pharmacology medicine.disease_cause Biochemistry Superoxide dismutase 03 medical and health sciences polycyclic compounds medicine cardiovascular diseases Viability assay Molecular Biology chemistry.chemical_classification Reactive oxygen species biology nutritional and metabolic diseases Cell Biology Molecular biology 030104 developmental biology chemistry Catalase Apoptosis Simvastatin biology.protein lipids (amino acids peptides and proteins) Oxidative stress medicine.drug |
Zdroj: | Journal of Investigative Dermatology. 137:1286-1296 |
ISSN: | 0022-202X |
Popis: | The prevention of hydrogen peroxide (H2O2)-induced oxidative stress has proved to be beneficial to vitiligo patients. Simvastatin possesses antioxidative capacity and has shown protective effect in various oxidative stress-related diseases. However, whether simvastatin can protect human melanocytes against oxidative stress has not been investigated. In this study, we initially found that pretreatment with 0.1 μmol/L to 1.0 μmol/L simvastatin led to increased cell viability and decreased cell apoptosis of melanocytes in response to H2O2. In addition, simvastatin was able to potentiate the activity of antioxidant enzymes and lessen intracellular reactive oxygen species accumulation. Furthermore, we found that simvastatin promoted the activation of nuclear erythroid 2-related factor (Nrf2) and that knockdown of Nrf2 abolished the protective effect of simvastatin against H2O2-induced oxidative damage. More importantly, the mutual enhancement between mitogen-activated protein kinase pathways and p62 contributed to simvastatin-induced Nrf2 activation in melanocytes. Finally, simvastatin showed more antioxidative capacity and better protective effect than aspirin in H2O2-treated melanocytes. Taken together, our results show that simvastatin protects human melanocytes from H2O2-induced oxidative stress by activating Nrf2, thus supporting simvastatin as a potential therapeutic agent for vitiligo. |
Databáze: | OpenAIRE |
Externí odkaz: |