Phytochrome Regulation of Seed Germination
| Autor: | Kijong Song, Giltsu Choi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Phytochrome biology Seed dormancy food and beverages biology.organism_classification 01 natural sciences Endosperm Agar plate 03 medical and health sciences Horticulture 030104 developmental biology Germination Arabidopsis Radicle 010606 plant biology & botany |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493996117 |
| DOI: | 10.1007/978-1-4939-9612-4_12 |
| Popis: | Seed germination assays consist of counting the number of germinated seeds, defined as seeds in which the radicle has ruptured the endosperm and emerged from the seed coat. In Arabidopsis seed germination assays, Arabidopsis seeds are surface-sterilized, plated on agar plates containing test compounds, and incubated at specific temperatures under specific light conditions, after which the germinated seeds are counted, either with the naked eye or under a microscope. This chapter describes step-by-step protocols for Arabidopsis seed germination assays under phytochrome-dependent conditions. |
| Databáze: | OpenAIRE |
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