Preparation of Nanometer Chitosan - RNA Composite and the Role in Induced Type I Interferon
| Autor: | Li Hui Zhao, De Ming Han, He Nan Zhao |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Materials science
viruses Cell technology industry and agriculture General Engineering RNA macromolecular substances Molecular biology Chitosan Small hairpin RNA chemistry.chemical_compound medicine.anatomical_structure chemistry Interferon Gene expression medicine Biophysics Bacterial outer membrane Intracellular medicine.drug |
| Zdroj: | Advanced Materials Research. 1051:43-47 |
| ISSN: | 1662-8985 |
| Popis: | This paper used double step compounding chitosan nanoparticles as carrier, took the virus's outer membrane NS1 shRNA delivery to cells,and simultaneous detection and characterization of chitosan-RNA Nanocomposites.The experiment results show that properties of chitosan-RNA nanocomposite is stable, using chitosan-RNA Nanocomposites as carrier carry shRNA transfecting B16-Blue INF-alpha/beta cells, shRNA can’t degradation by nuclear enzyme, improve cell absorbing and shRNA intracellular release.Experiments further confirmed that the chitosan - RNA Nanocomposites into the cell, NS1 shRNA is identified by RIG-I gene, induces gene expression of interferon, produces interferonItype, offers a new way for the application of new type of flu vaccine. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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