Molecular cloning and characterization of oocyte-specific Pat1a inRana rugosafrogs
| Autor: | Shoichi Kitahara, Maho Kodama, Takehiro Iwasaki, Yoshinobu Uno, Kazuhiro Saotome, Akira Oike, Yukiko Nakajima, Masahisa Nakamura, Yoichi Matsuda, Yosuke Umei, Yoriko Nakamura |
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| Rok vydání: | 2015 |
| Předmět: |
Gonad
medicine.diagnostic_test biology Physiology Xenopus Anatomy Oocyte biology.organism_classification Andrology medicine.anatomical_structure Complementary DNA Gene expression Genetics medicine Animal Science and Zoology Rana rugosa Molecular Biology Gene Ecology Evolution Behavior and Systematics Fluorescence in situ hybridization |
| Zdroj: | Journal of Experimental Zoology Part A: Ecological Genetics and Physiology. |
| ISSN: | 1932-5223 |
| Popis: | The Pat1 gene is expressed in the immature oocytes of Xenopus, and is reportedly involved in regulating the translation of maternal mRNAs required for oocyte-maturation. However, it is still unknown when Pat1a first appears in the differentiating ovary of amphibians. To address this issue, we isolated the full-length Pat1a cDNA from the frog Rana rugosa and examined its expression in the differentiating ovary of this frog. Among eight different tissues examined, the Pat1a mRNA was detectable in only the ovary. When frozen sections from the ovaries of tadpoles at various stages of development were immunostained for Vasa—a germ cell-specific protein—and Pat1a, Vasa-immunopositive signals were observed in all of the germ cells, whereas Pat1a signals were confined to the growing oocytes (50–200 μm in diameter), and absent from small germ cells ( |
| Databáze: | OpenAIRE |
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