Lipid accumulation during nitrogen and sulfur starvation in Chlamydomonas reinhardtii overexpressing a transcription factor

Autor:	Ruth Elena Soria-Guerra, Omar González-Ortega, Alejandro Rocha-Uribe, Sergio Rosales-Mendoza, Rodrigo Camarena-Rangel, Ana Erika Ochoa-Alfaro, Carlos Joel Salas-Montantes, Luz María Teresita Paz-Maldonado
Rok vydání:	2018
Předmět:	0301 basic medicine chemistry.chemical_classification biology Strain (chemistry) Transgene Chlamydomonas reinhardtii Plant Science Aquatic Science biology.organism_classification 03 medical and health sciences 030104 developmental biology Enzyme chemistry Biochemistry Biodiesel production Acyltransferase Gene expression lipids (amino acids peptides and proteins) Transcription factor
Zdroj:	Journal of Applied Phycology. 30:1721-1733
ISSN:	1573-5176 0921-8971
DOI:	10.1007/s10811-018-1393-6
Popis:	Algal triacylglycerol biosynthesis is of increasing interest for potential biodiesel production. In this study, we investigated the lipids content and gene expression changes in Chlamydomonas reinhardtii strain overexpressing a Dof-type transcription factor in response to nutrient deficiency. The untransformed strain grew in TAP complete medium and accumulated only 4.1% dry cell weight of fatty acids; while in the transgenic strain, the total fatty acids content increased to 15.58 and 17.02% in medium without sulfur or nitrogen, respectively. The major fatty acids found in the stressed microalgae were palmitic, oleic (all strains), and linoleic (transgenic strain). Additionally, we observed that the transgenic strain does not have a defect in carbon partitioning but redirect the carbon flux from starch to lipids. Among the most interesting results in the transcriptional profile, we found an overexpression of the enzymes lecithin cholesterol acyltransferase, glycerol-3-phosphatase acyltransferase, and phosphatidylcholine-sterol O-aciltransferase. As observed in this study, the combination of strategies (overexpression of a transcription factor and nutrient deficit) increases not only the content of total lipids in transgenic cells compared to an untransformed strain but, also the proportion of specific fatty acids that are desirable for biodiesel production.
Databáze:	OpenAIRE
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