Expression of a single-chain human interleukin-12 gene in transgenic tobacco plants and functional studies
| Autor: | Miguel A. Gómez-Lim, Carmen Sánchez-Torres, Federico Avila-Moreno, Abel Gutiérrez-Ortega, Luis Jorge Saucedo-Arias |
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| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_treatment
Transgene Bioengineering T helper cell Biology Applied Microbiology and Biotechnology law.invention medicine.anatomical_structure Cytokine Cancer immunotherapy law Immunology Gene expression medicine Recombinant DNA Interleukin 12 Interferon gamma Biotechnology medicine.drug |
| Zdroj: | Biotechnology and Bioengineering. 85:734-740 |
| ISSN: | 0006-3592 |
| DOI: | 10.1002/bit.20027 |
| Popis: | Interleukin 12 (IL-12) is a key heterodimeric cytokine produced by a variety of antigen-presenting cells, including dendritic cells, macrophages, and B cells. It displays a potent array of biological activities affecting natural killer (NK) and T cells. These activities include promotion of cell-mediated or type 1 T helper cell responses (Th1). Due to that property, IL-12 has been employed in cancer immunotherapy, in mouse models of infectious diseases and in airway inflammation, and it may also have utility as a vaccine adjuvant. Transgenic plants are being used in many laboratories around the world for the production of therapeutically valuable proteins and as vehicles for oral vaccines. Here we present the expression of a single-chain human interleukin-12 in transgenic tobacco plants. The biological activity of plant-produced IL-12 was determined by interferon gamma (IFN-gamma) production by natural killer (NK) cells, and the level of production was comparable to that obtained with commercially available recombinant IL-12. The potential use of this recombinant protein is discussed. |
| Databáze: | OpenAIRE |
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