| Autor: |
Mattout, Anna, Aaronson, Yair, Sailaja, Badi, Edupuganti Raghu Ram, Arigela Harikumar, Jan-Philipp Mallm, Sim, Kae, Nissim-Rafinia, Malka, Supper, Emmanuelle, Prim Singh, Siu Sze, Gasser, Susan, Rippe, Karsten, Meshorer, Eran |
| Rok vydání: |
2015 |
| Předmět: |
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| DOI: |
10.6084/m9.figshare.c.3636818_d4.v1 |
| Popis: |
HP1β is diffuse in fully pluripotent cells. a R1 ESC colonies surrounded by MEFs used as a feeder layer for the ESCs and as a staining positive control were co-immunostained with DAPI, Nanog, and HP1β. Images with long and short exposures are shown for the HP1β staining. Scale bars = 25 μm. b R1 and E14 ESCs were co-immunostained with Nanog and HP1β. The ESC colony in the marked area is enlarged in the right panel. Scale bars = 25 μm. c Co-immunostaining with H3K9me3 and HP1β. The R1 ESC colony (from Fig. 3) is shown on the right for easier comparison. Scale bars = 25 μm. d Relative levels of HP1γ transcripts (Cbx3) following siRNA treatment measured by RT-qPCR. e, f Cell proliferation assays performed in triplicate in six-well plates. The graphs show the number of WT ESCs (e) and HP1β KO ESCs (f) treated with siRNA against HP1γ or control siRNA every 24 h. (PDF 190 kb) |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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