IMMOBILIZED α-AMYLASE WITH HIGH ACTIVITY

Autor:	Fumiharu Kokubun, Machiko Shimada, Yoshio Nakamura
Rok vydání:	1984
Předmět:	Materials science Immobilized enzyme biology Ionic bonding Substrate (chemistry) General Medicine chemistry.chemical_compound chemistry Covalent bond Polymer chemistry biology.protein Amylase Cellulose Acrylic acid Carbodiimide
Zdroj:	Sen'i Gakkaishi. 40:T406-T410
ISSN:	1884-2259 0037-9875
DOI:	10.2115/fiber.40.10_t406
Popis:	Acrylic acid grafted cellulose was used as insoluble carrier for immobilization of α-amylase. Amino group of enzyme was coupled with carboxyl group of acrylic acid grafted cellulose by water soluble carbodiimide. And the activity of immobilized α-amylase to soluble starch was compared with that of free enzyme. In order to obtain the immobilized enzyme with high activity, various methods were tried. One of them is three steps reaction; the first step is the formation of ionic bond between carboxyl group and amino group, the second step is the formation of covalent bond between the residual carboxyl group and amino group, and the third step is the removal of ionic bond. As the result, immobilized α-amylase with high activity was obtained. The activity was 65% of that of free α-amylase. The value is very high as compared with those by other methods as polymer was used as substrate.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::574b1a6facc9237e2687d4ef4897fd69 https://doi.org/10.2115/fiber.40.10_t406 Zobrazit plný text záznamu