Expression of COX – 2 in Oral Leukoplakia Using RT-PCR: A Molecular Study

Autor:	KS Nagesh, Asha R Iyengar, Deepa B Patil, Vidya G S, N Rakesh
Rok vydání:	2014
Předmět:	medicine.medical_specialty Pathology medicine.diagnostic_test business.industry Cancer medicine.disease Control subjects Gastroenterology Oral leukoplakia stomatognathic diseases Real-time polymerase chain reaction Internal medicine Gene expression Biopsy medicine business Gene Leukoplakia
Zdroj:	IOSR Journal of Dental and Medical Sciences. 13:43-47
ISSN:	2279-0853 2279-0861
DOI:	10.9790/0853-131214347
Popis:	Background: Oral leukoplakia is a potentially malignant disorder that in a small but significant proportion of cases changes into oral cancer. Literature review suggests the association of inflammatory mediator such as cyclooxygenase-2(COX-2) with oral cancer. Expression of COX-2 gene in the oral potentially malignant disorders and oral cancer may add on a light to the existing diagnostics and therapeutics of these oro-mucosal diseases. Aim: To compare the expression of COX-2 gene in oral leukoplakia with that of controls. Methodology: The present study comprised of 40 subjects. 20 subjects each in oral leukoplakia & controls. Tissue samples were collected at the time of biopsy for the clinically diagnosed cases of leukoplakia and also from control subjects and stored in RNAase free containers. RNA was isolated and COX-2 gene expression was noted through RT- PCR method. Results: Demographic details were expressed in mean±standard deviation. Mann-Whitney test was applied to correlate the COX-2 expression between the groups and other parameters. A statistically significant higher expression of COX-2 was found in oral leukoplakia (0.739±0.078) when compared to controls (0.138±0.103) with P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5749e3973723a68ea316a6e04e49f4ec https://doi.org/10.9790/0853-131214347 Zobrazit plný text záznamu