X-ray Photoelectron Spectroscopic and Transmission Electron Microscopic Characterizations of Bacteriophage–Nanoparticle Complexes for Pathogen Detection
| Autor: | Edward Sacher, Étienne Boulais, Michel Meunier, Rosemonde Mandeville, Nancy Tawil |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
Carboxylic acid technology industry and agriculture Nanoparticle Nanotechnology Polyethylene glycol Photochemistry Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry.chemical_compound General Energy chemistry X-ray photoelectron spectroscopy Colloidal gold Covalent bond PEG ratio Physical and Theoretical Chemistry Carbodiimide |
| Zdroj: | The Journal of Physical Chemistry C. 117:20656-20665 |
| ISSN: | 1932-7455 1932-7447 |
| DOI: | 10.1021/jp406148h |
| Popis: | We report the synthesis and characterization of gold–bacteriophage hybrids for biodetection purposes. The physical and optical properties of gold nanoparticles (AuNPs) and the biological features of the phages offer a multifunctional scaffold with great potential for nanotechnologically based biomedical applications. AuNPs, stabilized (PEGylated) using heterobifunctional polyethylene glycol (PEG), were coupled to methicillin-resistant S. aureus-specific phages. The PEG ligands contain a thiol group for stable anchoring to the gold surface and a terminal carboxylic acid group for further coupling to the outside of the PEG shell by carbodiimide chemistry. Transmission electron microscopic analysis showed that the NP–phage bioconjugates are highly stable, with a median diameter of 90 nm. X-ray photoelectron spectroscopy was used to chemically characterize the surfaces of the PEG-functionalized AuNPs, the bacteriophages, and the gold–phage hybrids. The role of the interface and the covalent coupling chemistry... |
| Databáze: | OpenAIRE |
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