Photoemission probes of catalysis of benzo[a]pyrene epoxide reactions in complexes with linear, double-stranded and closed-circular, single-stranded DNA

Autor:	Ann Milliman, Shigeyuki Urano, Sharon M. Fetzer, Pierre R. LeBreton, Chao Ran Huang, Harry L. Price
Rok vydání:	1993
Předmět:	Tris Stereochemistry Epoxide Phenol extraction General Chemistry Biochemistry Catalysis chemistry.chemical_compound Colloid and Surface Chemistry Reaction rate constant chemistry Benzo(a)pyrene Pyrene DNA Carcinogen
Zdroj:	Journal of the American Chemical Society. 115:7794-7805
ISSN:	1520-5126 0002-7863
DOI:	10.1021/ja00070a028
Popis:	Fluorescence intensity measurements of overall, pseudo-first-order rate constants for two epoxide-containing metabolites of benzo[R]pyrene (BP) were carried out in Tris, EDTA buffer (pH 7.3) without DNA, and in buffer with double-stranded calf thymus DNA (DS ctDNA) and with closed-circular, single-stranded viral M 13mp19 DNA (SS M13 DNA). Highly purified SS M13 DNA was employed in order to avoid polymeric contamination which is present in DNA samples obtained using a standard preparation method relying on phenol extraction and which influences results from measurements of DNA-ligand interactions. The BP metabolites examined were highly carcinogenic (±)-trans-7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo[α]pyrene (BPDE) and less genotoxic benzo[α]pyrene 4,5-oxide (BPO)
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5724b958d04b6f4cd80dd0ad810f4629 https://doi.org/10.1021/ja00070a028 Zobrazit plný text záznamu