Application of Celestine Blue B and Gallocyanine for Studying the Effect of Drugs on the Production of Reactive Oxygen and Halogen Species by Neutrophils
| Autor: | Oleg M. Panasenko, I. V. Gorudko, Daria V. Grigorieva, V. E. Reut, Alexey V. Sokolov |
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| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
Reactive oxygen species biology Hypochlorous acid Superoxide 010401 analytical chemistry chemistry.chemical_element 02 engineering and technology 021001 nanoscience & nanotechnology Condensed Matter Physics medicine.disease_cause 01 natural sciences Oxygen 0104 chemical sciences Respiratory burst chemistry.chemical_compound chemistry Myeloperoxidase Scopoletin biology.protein medicine 0210 nano-technology Spectroscopy Oxidative stress Nuclear chemistry |
| Zdroj: | Journal of Applied Spectroscopy. 87:693-700 |
| ISSN: | 1573-8647 0021-9037 |
| Popis: | We investigated the effect of drugs (dapsone, paracetamol, and isoniazid) on the production of reactive oxygen and halogen species by neutrophils. The standard fluorescent method using scopoletin as well as recently developed fluorescent methods using oxazine dyes, celestine blue B, and gallocyanine were employed for this purpose. Celestine blue B reacts selectively with hypochlorous acid, while gallocyanine reacts mainly with the superoxide radical-anion, which reveals the regulatory effect of anti-inflammatory drugs on neutrophil NADPH-oxidase and myeloperoxidase responsible for the production of reactive oxygen and halogen species, respectively. These results indicate that gallocyanine and celestine blue B hold promise as chemosensors for studying the effect of drugs used in anti-inflammatory therapy for the neutrophil respiratory burst. |
| Databáze: | OpenAIRE |
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