Cytologic detection of Toxoplasma gondii in the cerebrospinal fluid of a dog and in vitro isolation of a unique mouse-virulent recombinant strain
| Autor: | Waléria Borges-Silva, Gideão S Galvão, George Rêgo Albuquerque, M.M. Rezende-Gondim, Daniele de Santana Rocha, Luis Fernando Pita Gondim |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
General Veterinary biology 030231 tropical medicine Toxoplasma gondii Virulence medicine.disease biology.organism_classification Virology Toxoplasmosis Neospora caninum 030308 mycology & parasitology law.invention 03 medical and health sciences 0302 clinical medicine Cerebrospinal fluid law Cytology parasitic diseases medicine Recombinant DNA Parasite hosting |
| Zdroj: | Journal of Veterinary Diagnostic Investigation. 33:591-594 |
| ISSN: | 1943-4936 1040-6387 |
| Popis: | Parasites resembling Neospora caninum or Toxoplasma gondii were detected by cytologic examination of cerebrospinal fluid (CSF) from a dog with neurologic disease. The dog became severely ill and was euthanized. Canine tissue homogenates were used for direct parasite isolation in cell culture, bioassay in 2 mouse lineages, and PCR. T. gondii was isolated in monkey kidney cells, and species identity was confirmed by PCR. Inoculated parasites were highly virulent for mice, which developed clinical signs and were euthanized immediately. PCR-RFLP for T. gondii using the cultured isolate (TgDgBA22) was conducted with 12 genetic markers, and a unique recombinant strain was identified. Detection of T. gondii by CSF cytology, although described in humans, had not been reported previously in dogs, to our knowledge, and was crucial for the diagnosis of toxoplasmosis in the examined dog. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|