Improved Extraction of Ginkgotoxin (4′-O-methylpyridoxine) from Ginkgo biloba Products
| Autor: | Peter M. Scott, Guillaume A. Lawrence |
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| Rok vydání: | 2005 |
| Předmět: | |
| Zdroj: | Journal of AOAC INTERNATIONAL. 88:26-29 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.1093/jaoac/88.1.26 |
| Popis: | A simple extraction procedure was applied to the analysis of canned/packaged white nuts and Ginkgo biloba extracts. Extraction by shaking with water at room temperature was more convenient to use than a previously published Soxhlet procedure for analysis of packaged Ginkgo biloba seeds (white nuts) for ginkgotoxin; recoveries from spiked dried seeds by the simple extraction procedure averaged 76%. Determination was by liquid chromatography with UV or fluorescence detection. Recoveries of ginkgotoxin from a spiked and unspiked natural health product (powder from Ginkgo biloba capsules) were equivalent by both procedures; recovery from spiked powder by the simple extraction procedure was 81%. Application of this extraction procedure in the analysis of 6 samples of white nuts (vacuum packaged and canned products) showed that free ginkgotoxin was present in 5 samples at concentrations up to 25 μg/g dry weight. Total ginkgotoxin was determined after hydrolysis with β-glucosidase of sample extracts in which a peak corresponding to the 5′-O-glucoside was detected. Ginkgotoxin was determined in 10 Ginkgo biloba natural health products by the same method at levels up to 181 μg/g. |
| Databáze: | OpenAIRE |
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