| Autor: | M.E. Escobar, A.L. Stern, N. Adragna, S. Alonso-Romanowski, Maria C. Taira |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Liposome
food.ingredient Chromatography biology Chemistry technology industry and agriculture Serum albumin Albumin Bioengineering General Medicine Applied Microbiology and Biotechnology Lecithin food Biochemistry Yolk biology.protein medicine Dimyristoyl Lecithin lipids (amino acids peptides and proteins) Sodium nitroprusside Bovine serum albumin Biotechnology medicine.drug |
| Zdroj: | Biotechnology Letters. 25:1425-1429 |
| ISSN: | 0141-5492 |
| DOI: | 10.1023/a:1025051511608 |
| Popis: | A rapid determination of protein-liposome binding was developed to predict the circulation time of the system within an animal, which is a function of the amount and type of protein bound. The binding pattern of albumin to liposomes, with and without sodium nitroprusside (SNP), was analyzed by SDS-PAGE. Liposomes were made of egg yolk lecithin, soybean lecithin and dimyristoyl lecithin, and contained SNP. They bound 58%, 26% and 100% bovine serum albumin, respectively, when compared to their corresponding controls lacking SNP. The method applied is simpler and significantly faster than ordinary chemical determinations. |
| Databáze: | OpenAIRE |
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