Accelerated construction of a regional DNA-barcode reference library: caddisflies (Trichoptera) in the Great Smoky Mountains National Park
| Autor: | Paul D. N. Hebert, Oliver S. Flint, David E. Ruiter, David A. Etnier, Charles R. Parker, Christy J. Geraci, Luke M. Jacobus, R. Edward DeWalt, Jason L. Robinson, Xin Zhou |
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| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | Journal of the North American Benthological Society. 30:131-162 |
| ISSN: | 1937-237X 0887-3593 |
| DOI: | 10.1899/10-010.1 |
| Popis: | Deoxyribonucleic acid (DNA) barcoding is an effective tool for species identification and life-stage association in a wide range of animal taxa. We developed a strategy for rapid construction of a regional DNA-barcode reference library and used the caddisflies (Trichoptera) of the Great Smoky Mountains National Park (GSMNP) as a model. Nearly 1000 cytochrome c oxidase subunit I (COI) sequences, representing 209 caddisfly species previously recorded from GSMNP, were obtained from the global Trichoptera Barcode of Life campaign. Most of these sequences were collected from outside the GSMNP area. Another 645 COI sequences, representing 80 species, were obtained from specimens collected in a 3-d bioblitz (short-term, intense sampling program) in GSMNP. The joint collections provided barcode coverage for 212 species, 91% of the GSMNP fauna. Inclusion of samples from other localities greatly expedited construction of the regional DNA-barcode reference library. This strategy increased intraspecific diver... |
| Databáze: | OpenAIRE |
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