The Novel Antihyperglycaemic Action of Hunteria umbellate Seed Fractions Mediated Via Intestinal Glucose Uptake Inhibition
| Autor: | E O Agbaje, Adejuwon Adewale Adeneye, O. O. Adeyemi, Margaret O. Sofidiya |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
Chloroform biology Glucose uptake Alkaloid Butanol Ethyl acetate biology.organism_classification Hunteria umbellata chemistry.chemical_compound Endocrinology Complementary and alternative medicine Mechanism of action Distilled water chemistry Internal medicine Drug Discovery medicine medicine.symptom |
| Zdroj: | African Journal of Traditional, Complementary and Alternative Medicines. 9 |
| ISSN: | 0189-6016 |
| DOI: | 10.4314/ajtcam.v9i1.3 |
| Popis: | The present study evaluated the antihyperglycaemic effect and mechanism of action of fractions of the aqueous seed extract of Hunteria umbellata (K. Schum.) Hallier f. ( HU ) in normal and alloxan-induced hyperglycaemic rats. HU was partitioned in chloroform, acetyl acetate and butan-1-ol to give chloroform fraction ( HU c), ethyl acetate fraction ( HU e), butanol fraction ( HU b) and the “residue” ( HU m), respectively. 200 mg/kg of each of these fraction dissolved in 5% Tween 20 in distilled water was investigated for its acute oral hypoglycaemic effects in normal rats over 6 hours while its repeated dose antihyperglycaemic effect was evaluated in alloxan-induced hyperglycaemic rats over 5 days. In addition, 50 mg/kg of the crude alkaloid fraction ( HU Af) extracted from HU was evaluated for its possible antihyperglycaemic activity in alloxaninduced hyperglycaemic rats using oral glucose tolerance test (OGTT) over 6 hours. Using the solvent system, distilled water-butanol-ammonium hydroxide (2:15:1, v/v/v), HU b was chromatographed and stained with Dragendorff’s reagent for confirmatory qualitative analysis for alkaloids. Results showed that oral pre-treatment with 200 mg/kg of HU e, HU b and HU m resulted in a significant (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|