Immunolocalization and serum antibody responses to Brugia malayi pepsin inhibitor homolog (Bm-33)

Autor: Srinivasan Dharanya, Chandanpurath Shiny, Sridharan Aishwarya, Arivazhagan Sindhu, N.S.A. Krushna, Rangarajan Badri Narayanan
Rok vydání: 2009
Předmět:
Zdroj: Microbiology and Immunology. 53:173-183
ISSN: 1348-0421
0385-5600
DOI: 10.1111/j.1348-0421.2009.00114.x
Popis: cDNA coding for Brugia malayi pepsin inhibitor homolog (Bm-33) from the human filarial parasite was cloned in pRSET for large-scale expression and functional characterization. The pRSET-B cloned gene did not yield recombinant protein expression and the reason was attributed to the presence of an N-terminal signal peptide. The gene was subcloned in pRSET-A without a signal peptide and the 33 kDa histidine-tagged recombinant protein was purified by IMAC. All individuals from an endemic area generated IgG responses against Bm-33 in the order MF>CP>EN. Isotype analysis indicated an elevated IgG4 reactivity in the order MF>EN>CP. Bm-33-specific IgE levels were elevated in MF, CP and EN compared to non-endemic normals with no significant differences among the groups. Paraffin-embedded sections of Setaria digitata (cattle filarial parasite) stained with mouse anti-Bm-33 antibodies exhibited the hypodermal nature of Bm-33. These findings suggest that Bm-33 is an immunodominant antigen and contributes to filarial pathogenesis.
Databáze: OpenAIRE
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