Structural modification of alfalfa stems during hot water and enzymatic hydrolysis for sugar production

Autor:	Andrea Facchini, Daniele Dondi, Paolo Arcioni, Marco Malagodi, Alberto Zeffiro, Paola Nola, Armando Buttafava, Erik Nielsen, Maurizio Licchelli
Rok vydání:	2015
Předmět:	Chromatography Materials science Polymers and Plastics biology food and beverages Forage Cellulase Furfural chemistry.chemical_compound chemistry Biochemistry Biofuel Enzymatic hydrolysis biology.protein Bioorganic chemistry Medicago sativa Hydroxymethylfurfural
Zdroj:	Cellulose. 22:1853-1860
ISSN:	1572-882X 0969-0239
Popis:	Bioethanol production from non-food lignocellulosic material is one of the most important topics in the field of biofuel production. We report on experiments with a treatment for the production of fermentable sugars from the fibrous parts of alfalfa (Medicago sativa L.), a widely used forage plant. The process is divided into several consecutive steps: ball milling, microwave assisted pretreatment in aqueous medium and enzymatic hydrolysis with a mixture of cellulase and β-glucosidase leading to the final production of fermentable sugars. This process was followed by a detailed HPLC analysis for the detection of sugars, oligomers and by-products, such as furfural, hydroxymethylfurfural and acids. Scanning electron microscopy was used for a visual characterization of the material throughout all these steps. Several plant structures were identified and their successive changes during the different treatment steps were monitored and followed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::56c7819b3a710deeb4e9fe701ff80d55 https://doi.org/10.1007/s10570-015-0580-8 Zobrazit plný text záznamu Full text from SpringerLink