| Autor: |
M. Puygrenier, J.C. Gautier, Patrick Couvreur, J.L. Grangier |
| Rok vydání: |
1991 |
| Předmět: |
|
| Zdroj: |
Journal of Controlled Release. 15:3-13 |
| ISSN: |
0168-3659 |
| DOI: |
10.1016/0168-3659(91)90098-x |
| Popis: |
This study presents a useful approach for the identification of factors which must be considered to permit the development of successful nanoparticle growth hormone releasing factor (GRF) formulation. The results show that GRF can be associated with biodegradable poly(alkylcyanoacrylate) nanoparticles. Among other parameters, the moment at which GRF was incorporated in the polymerization medium was found to be a critical factor in avoiding the degradation of GRF and the covalent bonding of the peptide with the polymer. The drugpolymer interaction was, however, confirmed by gel permeation chromatography (GPC), showing the appearance of a heavier polymeric fraction when GRF was added at the very beginning of the polymerization process. Drug-release experiments suggested that the liberation of GRF from nanoparticles resulted rather from the bioerosion of the polymeric matrix than from the passive diffusion of the peptide through the polymer. Indeed, drug-release appeared dependent upon the esterase content of the incubation medium. Finally, it was found that the alkyl chain length of the cyanoacrylic polymer was able to modulate the rate of GRF release. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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