Autor: R. Eric Cerny, Timothy W. Conner, Shihshieh Huang, Jintai Huang, Brandon J. Fabbri, Youlin Qi, Lyle Crossland, Jennifer Listello, Carrie M. Aydt
Rok vydání: 2003
Předmět:
Zdroj: Plant Molecular Biology. 52:357-369
ISSN: 0167-4412
DOI: 10.1023/a:1023953130574
Popis: We have demonstrated that RNA-binding proteins from coliphages and yeast can function as translational repressors in plants. RNA sequences called translational operators were inserted at a cap-proximal position in the 5′-UTR of mRNAs of two reporter genes, gusor aroA:CP4. Translation of the reporter mRNAs was efficiently repressed when the RNA binding protein that specifically binds to its cognate operator was co-expressed. The efficiency of translational repression by RNA-binding protein positively correlated with the amount of binding protein in transformed plant cells. Detailed studies on coliphage MS2 coat protein-mediated translational repression also suggested that the efficiency of translational repression was position-dependent. A translational operator situated at the cap-proximal position was more efficient in conferring repression than one that was placed cap-distal. Translational repression can be an efficient means for regulation of transgene expression, thereby broadening current approaches for transgene regulation in plants.
Databáze: OpenAIRE
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