Measuring zinc in living cells

Autor: John H. Weiss, Z.-L Zhou, Dien Ton-That, Stefano L. Sensi, Kyle R. Gee
Rok vydání: 2002
Předmět:
Zdroj: Cell Calcium. 31:245-251
ISSN: 0143-4160
DOI: 10.1016/s0143-4160(02)00053-2
Popis: New fluorescent indicators with nanomolar to micromolar affinities for Zn(2+) have been synthesized in wavelengths from UV to the far red. The UV light-excited indicators are ratiometric. The visible wavelength indicators are non-ratiometric and exhibit large and pH-independent fluorescence increases with increasing zinc concentrations, with little to no sensitivity to physiologically relevant Ca(2+) concentrations. Experiments in neuronal and non-neuronal cell cultures show the new indicators to retain their sensitivity to and selectivity for zinc after conversion to cell-permeable forms.
Databáze: OpenAIRE
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