Luteal Enzymes of the Luteinizing Hormone and β-Adrenergic Signal Transduction Pathways in Hypophysectomized Rabbits do not Require Pituitary Hormone Support1
| Autor: | P L Keyes, Mary Hunzicker-Dunn, Charles H. Bill, John E. Gadsby, Josephine B. Miller, A Chen, Andrew R. Labarbera, V Jackiw |
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| Rok vydání: | 1991 |
| Předmět: |
Pituitary gland
medicine.medical_specialty Cell Biology General Medicine Luteal phase Biology Adenylyl cyclase chemistry.chemical_compound medicine.anatomical_structure Endocrinology Reproductive Medicine chemistry Internal medicine medicine CAMP binding Protein kinase A Luteinizing hormone Corpus luteum Hormone |
| Zdroj: | Biology of Reproduction. 44:609-619 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod44.4.609 |
| Popis: | Experiments were conducted to determine whether continuous pituitary hormone support is required for expression of the LH and beta-adrenergic cAMP signal transduction pathways in rabbit CL during pseudopregnancy. Parameters of the LH and catecholamine cAMP signal transduction pathways in CL of estrogen-treated hypophysectomized rabbits were compared to those of pituitary-intact rabbits. Results showed that each of the parameters of the LH and beta-adrenergic cAMP signal transduction pathways was retained in CL taken from estrogen-treated pseudopregnant rabbits that had been hypophysectomized for as long as 13 days at levels not significantly different from those of estrogen-treated pituitary-intact rabbits. These included luteal basal, and LH-, epinephrine-, and fluoride-stimulated adenylyl cyclase activities; total luteal cAMP levels; the number and affinity of cAMP-dependent protein kinase regulatory subunit cAMP binding sites; binding activity of the type I and type II regulatory subunits; and the amount of catalytic subunit protein of cAMP-dependent protein kinase. We conclude that expression of the proteins of the cAMP signal pathway for LH and beta-adrenergic hormones in CL of estrogen-treated rabbits does not require pituitary hormone support. |
| Databáze: | OpenAIRE |
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