Spike Activity and Genome Instability in Neurons of the Amygdaloid Complex in Rats of Selected Strains with Contrasting Nervous System Arousability in Normal Conditions and Stress

Autor: O. A. Lyubashina, Ivan B. Sivachenko, M. B. Pavlova, N. A. Dyuzhikova, S. S. Panteleev, A. I. Vaido, N. V. Shiryaeva
Rok vydání: 2021
Předmět:
Zdroj: Neuroscience and Behavioral Physiology. 51:620-628
ISSN: 1573-899X
0097-0549
DOI: 10.1007/s11055-021-01115-0
Popis: Neuron activity in the amygdaloid complex was studied in baseline conditions and on exposure to electrical stimulation of the prefrontal cortex in intact rats and rats subjected to prolonged emotional/pain stress (PEPS) using rats of bred strains with high (HT) and low (LT) thresholds of nervous system arousability; levels of genome instability were also studied in amygdaloid complex cells using a protein marker for double-stranded DNA breaks, i.e., histone H2AX phosphorylated at serine 139 (γ-H2AX phospho Ser139). We report the first finding of a direct link between basal (baseline) spike activity in amygdaloid complex neurons and their genome instability level, along with a relationship between basolateral amygdala neuron responses to excitatory stimulation of the infralimbic cortex and the innate genetically determined level of nervous system arousability. PEPS enhanced the functional activity of the amygdaloid complex in HT rats in terms of the baseline neuron spike activity frequency, mean spike number, and latency in responses to cortical stimulation. LT rats in the same conditions showed more significant increases in baseline neuron activity frequency, accompanied by increases in immunoreactivity of cells to γ-H2AX phospho Ser139 and a significant reduction in the latency of the responses of amygdalar neurons to cortical stimulation with no change in the number of spikes in responses.
Databáze: OpenAIRE
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