DNA barcoding in medicinal plants: Testing the potential of a proposed barcoding marker for identification of Uncaria species from China

Autor:	Xiao Jun Ma, Hai-Tao Li, Ying Feng Niu, Yan Hong Guan, Zhong Lian Zhang, Mei Fang Song, Li-Xia Zhang
Rok vydání:	2015
Předmět:	Range (biology) Computational biology Biology biology.organism_classification Biochemistry DNA barcoding DNA sequencing Uncaria Genus Botany Identification (biology) Medicinal plants Ecology Evolution Behavior and Systematics Molecular identification
Zdroj:	Biochemical Systematics and Ecology. 60:8-14
ISSN:	0305-1978
DOI:	10.1016/j.bse.2015.02.017
Popis:	DNA barcoding, an increasingly popular mean of species identification, has been widely used for global species identification despite a consensus not being reached regarding which DNA sequences can be used as the best plant barcodes. In this study, we tested the feasibility of five candidate DNA barcodes (nrITS, nrITS2, matk, rbcL and trnH-psbA) for identifying Uncaria species. We collected a total of 54 specimens of 10 Uncaria species across its distributional range. BLAST, barcoding gaps, tree-based methods and TAXONDNA analysis were used to investigate the molecular identification capability of the candidate DNA barcodes. The results showed that the ITS2 is most suitable as a candidate DNA barcode for identification of medicinal plants of the genus Uncaria.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::567e17f4873ad585b5ac8190f4ed1225 https://doi.org/10.1016/j.bse.2015.02.017 Zobrazit plný text záznamu Full Text from ScienceDirect