Mechanisms of mild hyperthermia-induced cytotoxicity in human prostatic carcinoma cells: Perturbation of cell cycle progression and DNA fragmentation
| Autor: | Sang H. Kim, Peter M. Corry, Charles A. Vidair, Jae H. Kim, Yong J. Lee, Elwood P. Armour, Samuel Ryu |
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| Rok vydání: | 1994 |
| Předmět: |
Gel electrophoresis
Hyperthermia Programmed cell death Pathology medicine.medical_specialty Physiology Biology medicine.disease Biochemistry Mild hyperthermia Apoptosis Carcinoma medicine Cancer research DNA fragmentation General Agricultural and Biological Sciences Cytotoxicity Developmental Biology |
| Zdroj: | Journal of Thermal Biology. 19:305-313 |
| ISSN: | 0306-4565 |
| DOI: | 10.1016/0306-4565(94)90066-3 |
| Popis: | 1. 1.|We investigated the relationship between heat-induced perturbation of cell cycle progression and cytotoxicity at low temperature hyperthermia in three human cell lines human breast carcinoma cells (MCF-7) and human prostatic adenocarcinoma cells (DUT-145 and PC-3). 2. 2.|MCF-7 cells which were resistant to mild hyperthermia exhibited a G 1 block during heating at 41°C. In contrast, DUT-145 and PC-3 cells which were sensitive to mild hyperthermia failed to accumulate in G 1 -phase. Both DUT-145 and PC-3 cells progressed through S-phase and accumulated in the G 2 /M-phase. These results rule out any species-based generalization for heat sensitivity. 3. 3.|Electron micrographs and gel electrophoresis data show the accumulation of mitotic-like cells and DNA fragmentation during mild hyperthermia in prostatic carcinoma cells. 4. 4.|These results suggest that cell death is associated, at least partly with DNA fragmentation, perhaps apoptosis in prostatic carcinoma cells. |
| Databáze: | OpenAIRE |
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