Diagnosis of Trichomonas vaginalis using real-time polymerase chain reaction (PCR) among women at Institut Pasteur of Cte dIvoire
| Autor: | T. Blavo-Kouamé, K. E. Angora, A. Yéo, A. Ouattara, A. Ira-Bonouman, A. T. Offianan, J. Ouhon, H. Faye-Ketté |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Sexually transmitted disease Trichomoniasis 030106 microbiology Prevalence Plant Science Biology medicine.disease_cause medicine.disease Microbiology Virology Giemsa stain Staining law.invention 03 medical and health sciences 0302 clinical medicine Infectious Diseases Real-time polymerase chain reaction law medicine Trichomonas vaginalis 030212 general & internal medicine Polymerase chain reaction |
| Zdroj: | African Journal of Microbiology Research. 12:988-993 |
| ISSN: | 1996-0808 |
| DOI: | 10.5897/ajmr2018.8989 |
| Popis: | Trichomoniasis is a sexually transmitted disease caused by a mobile flagellate pathogenic protist protozoan of the urogenital tract, Trichomonas vaginalis. No data is available in Cote d’Ivoire about its diagnosis by molecular methods. This study aims to identify T. vaginalis by polymerase chain reaction (PCR) among women at Institut Pasteur of Cote d’Ivoire. Vaginal swabs were obtained from each woman from July to October, 2013. For T. vaginalis detection, Giemsa stain for microscopic examination and real-time PCR were performed. The PCR targeted 67 bp region of a repeated sequence of the T. vaginalis genome. A positive T. vaginalis result was defined as a cycle threshold (Ct) less than 36. The results show that of the 194 specimens tested by both Giemsa stain method and Real-time PCR, 2 were positive to Giemsa stain (1.03%) and 7 were positive in PCR assay (3.61%). Women who had multiple sexual partners in the last two months were found to be the most infected. Comparing the two methods, real-time PCR had a higher sensitivity (100%) and specificity (97.40%). This study shows that the prevalence rate of T. vaginalis infection remains low. However, using the PCR approach allows for better detecting infection than conventional staining method. Key words: Trichomonas vaginalis, real-time polymerase chain reaction (PCR), Giemsa stain, Cote d’Ivoire. |
| Databáze: | OpenAIRE |
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