DETERMINATION OF FLUOROQUINOLONE ANTIBACTERIALS IN PHARMACEUTICAL FORMULATIONS BY CAPILLARY ELECTROPHORESIS

Autor:	An-Cheng Wu, Shao-Wen Sun
Rok vydání:	1999
Předmět:	Chromatography Chemistry Clinical Biochemistry Pharmaceutical Science Biochemistry Pefloxacin Dosage form Analytical Chemistry Sparfloxacin Capillary electrophoresis medicine Enoxacin Lomefloxacin Ofloxacin medicine.drug Antibacterial agent
Zdroj:	Journal of Liquid Chromatography & Related Technologies. 22:281-296
ISSN:	1520-572X 1082-6076
DOI:	10.1081/jlc-100101660
Popis:	To meet the requirement of quantitative analysis, this work re-investigated the parameters relevant to the separation of seven fluoroquinolone antibacterials, ciprofloxacin, enoxacin, lomefloxacin, norfloxacin, ofloxacin, pefloxacin, and sparfloxacin, based on the method developed previously in our laboratory. With the optimum conditions obtained: electro- phoretic media - 65 mM sodium borate, 35 mM sodium dihydrogen phosphate, 60 mM sodium cholate, pH 7.3 / acetonitrile (72 : 28, v/v); fused silica capillary - 50 μm I.D., total length 67 cm, detection length 52 cm; hydrodynamic injection - 60 mbar, 3 sec; voltage - 27 kV; temperature - 25°C and detection wavelength - 275 nm, the fluoroquinolones were well separated within 8.5 min. After being validated the method was used for the assay of the formulation products. The assay results were within 95∼105 % of their label claims. The method should be applicable to the analysis of these and other similar formulation products containing these drugs.
Databáze:	OpenAIRE
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