Autor: Justin K. M. Roberts, Jean-Luc Le Bail, Marina Gromova, Sandra Cortès, Claude Roby
Rok vydání: 2002
Předmět:
Zdroj: Molecular Biology Reports. 29:145-149
ISSN: 0301-4851
DOI: 10.1023/a:1020309309045
Popis: Sucrose is the cornerstone of higher plant metabolism. Produced by photosynthesis, sucrose is the main substrate for respiration and biosynthesis. The emerging idea is that sucrose may act as regulator of its own metabolism, characterized in particular by a permanent process of degradation and formation. This sucrose turnover may control several important physiological functions. Of particular concern is an energy dependent cycle involving the hexokinase. This report presents an experimental approach to define quantitatively physiological states of suspension-cultured plant cells wih reference to their sucrose content and respiration rate. Sucrose depletion of normal cells incubated in a medium devoid of sugar is measured in vivo using 13C and respiration is simultaneously recorded. Results obtained with sucrose-storing cells and Arabidopsis thaliana show that respiration rate is closely linked to the available sucrose. Sucrose-depleted cells offer a stable model to study the bioenergetics of the process.
Databáze: OpenAIRE
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