Preparation of a bi-hapten antigen and the broad-specific antibody for simultaneous immunoassay of penicillins and tetracyclines in milk

Autor:	Jian Ping Wang, Jing Liu, Yi Fen Zhang, Guo Xian Zhao, Sai Nan Jiao
Rok vydání:	2012
Předmět:	Chlortetracycline Immunogen Chromatography medicine.diagnostic_test biology Immunology Oxytetracycline Penicillin chemistry.chemical_compound chemistry Immunoassay Sulbenicillin medicine biology.protein Bovine serum albumin Agronomy and Crop Science Hapten Food Science medicine.drug
Zdroj:	Food and Agricultural Immunology. 23:273-287
ISSN:	1465-3443 0954-0105
DOI:	10.1080/09540105.2011.624175
Popis:	A novel bi-hapten immunogen was prepared by coupling amoxicillin and chlortetracycline to bovine serum albumin in turn. Then the immunogen was used to immunise New Zealand rabbits to produce the polyclonal antibody. The obtained antibody simultaneously cross-reacted with six penicillins (amoxicillin, penicillin G, ampicillin, penicillin V, carbencillin and sulbenicillin) and four tetracyclines (tetracycline, oxytetracycline, doxycycline and chlortetracycline) with crossreactivities in a range of 24–100%. Then a broad specificity enzyme linked immunosorbent assay was optimised for the simultaneous detection of the 10 drugs in milk. The limits of detection for these analytes were in a range of 0.4–3.7 ng/mL depending on the compound. The intra- and inter-assay recoveries of the 10 analytes from standards fortified blank milk ranged from 80.8 to 99.4% with coefficients of variation lower than 13%. This study provided a new strategy for the development of multi-analyte immunoassay through immunogen d...
Databáze:	OpenAIRE
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