Precipitating Antibody Production in Germfree Chickens
| Autor: | B. S. Wostmann, G. B. Olson |
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| Rok vydání: | 1964 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 92:41-48 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.92.1.41 |
| Popis: | Summary Germfree and conventional chickens depicted three differences in their antibody response to a single sensitizing injection of BSA: (1) germfree chickens seem to require 1 more day to produce a detectable circulating precipitating antibody, (2) both groups reach their peak antibody production on the 8th day after sensitization, but the germfree chicken had approximately one-half the amount of circulating antibody in comparison to the conventional chicken, (3) the germfree group maintained a proportional higher level of antibody after day of peak production than did the conventional bird. Possible explanations for these differences are presented for consideration. Electrophoretic studies of the antiplasmas showed a parallel increase in the γ-globulin region, which on the 8th day after antigen injection was approximately 300 to 400 mg/100 ml over normal base levels for the two groups, and then subsequently returned toward the normal. A determination of the half-life of the γ-globulin fraction of the germfree and conventional animals depicted no difference in the metabolic degradation of the protein. It appears that even with the quantitative differences in the RES of the germfree chicken it is still capable of producing an effective response to an antigenic stimulation. |
| Databáze: | OpenAIRE |
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