Different factors affecting polyAT conformation in microemulsions: effects of variable P0 and KCl concentration

Autor:	Licia Scibetta, Anna Maria Giuliani, C. Andrea Boicelli, Marta Airoldi, Mauro Giustini, Marcello Giomini, G. Gennaro
Rok vydání:	2002
Předmět:	chemistry.chemical_classification Chromatography Chemistry Cationic polymerization Inner core Analytical chemistry General Physics and Astronomy Dielectric Polymer Volume (thermodynamics) Polynucleotide Denaturation (biochemistry) Microemulsion Physical and Theoretical Chemistry
Zdroj:	Physical Chemistry Chemical Physics. 4:3859-3864
ISSN:	1463-9084 1463-9076
DOI:	10.1039/b202839e
Popis:	The behaviour of the synthetic polynucleotide polyAT entrapped in the inner core of a quaternary cationic microemulsion has been investigated by means of CD and UV spectroscopies in the presence of KCl (variable temperature at constant W0 = [H2O]/[CTAB] and P0 = [cosurfactant]/[CTAB]) and NaCl (variable P0 at constant W0 and temperature). While the presence of KCl gives rise to effects which are not substantially different from those already reported for NaCl (absence of thermal melting and formation of compact ψ(−) structures), the peculiar results obtained as a consequence of the progressive addition of cosurfactant (denaturation of the polymer at low [NaCl] and W0; transition from B- to A-form at intermediate [NaCl], formation of ψ(−) aggregates at high [NaCl]) can be attributed to the characteristics of the microemulsive system (restricted volume), of the micellar water (low dielectric constant and reduced activity) and of the interface (progressively higher cosurfactant concentration).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::560ce74da6ecebea179d5259aa284ce5 https://doi.org/10.1039/b202839e Zobrazit plný text záznamu