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The purpose of this study was to analyze the difference between male and female tennis players` two-handed backhand stroke and to provide basic data which helps precise and efficient instruction for the sake of precise postures, enhanced performances and skills. 5 male and 5 female university players were recruited as subjects, and the mean difference between the kinematic variables such as the time from backswing to impact and total swing time, racket head velocity, change of the center of body gravity in two-handed backhand stroke through three-dimensional motion analysis. The test data was analyzed by t-test, and the alpha level of |