Evaluation of Hepatocyteprotective and Anti-hepatitis B Virus Properties of Cichoric Acid from Cichorium intybus Leaves in Cell Culture
| Autor: | Tao Liu, Xiao-ping Yu, Hong-Li Zhang, Yong-Yong Zhang, Jun Zhao, Rong-Fa Guan, Ling-hao Dai, Yi-Hang Wu |
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| Rok vydání: | 2014 |
| Předmět: |
Pharmacology
Hepatitis B virus Liver infection biology Cichoric acid Duck hepatitis B virus Pharmaceutical Science General Medicine Hepatitis B medicine.disease_cause medicine.disease biology.organism_classification Virology Molecular biology Virus chemistry.chemical_compound chemistry Cichorium medicine Caffeic acid |
| Zdroj: | Biological and Pharmaceutical Bulletin. 37:1214-1220 |
| ISSN: | 1347-5215 0918-6158 |
| DOI: | 10.1248/bpb.b14-00137 |
| Popis: | Hepatitis B is the most common serious liver infection in the world. To date, there is still no complete cure for chronic hepatitis B. Natural caffeic acid analogues possess prominent antiviral activity, especially anti-hepatitis B virus (HBV) and anti-human immunodeficiency virus effects. Cichoric acid is a caffeic acid derivative from Cichorium intybus. In the study, the anti-hepatitis B property of cichoric acid was evaluated by the D-galactosamine (D-GalN)-induced normal human HL-7702 hepatocyte injury model, the duck hepatitis B virus (DHBV)-infected duck fetal hepatocytes and the HBV-transfected cell line HepG2.2.15 cells, respectively. The results showed that cichoric acid attenuated significantly D-GalN-induced HL-7702 hepatocyte injury at 10-100 µg/mL and produced a maximum protection rate of 56.26%. Moreover, cichoric acid at 1-100 µg/mL inhibited markedly DHBV DNA replication in infected duck fetal hepatocytes. Also, cichoric acid at 10-100 µg/mL reduced significantly the hepatitis B surface and envelope antigen levels in HepG2.2.15 cells and produced the maximum inhibition rates of 79.94% and 76.41%, respectively. Meanwhile, test compound at 50-100 µg/mL inhibited markedly HBV DNA replication. In conclusion, this study verifies the anti-hepatitis B effect of cichoric acid from Cichorium intybus leaves. In addition, cichoric acid could be used to design the antiviral agents. |
| Databáze: | OpenAIRE |
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