Electrochemiluminescence Detection of c-Myc mRNA in Breast Cancer Cells on a Wireless Bipolar Electrode

Autor:	Jing-Juan Xu, Guang-Sheng Qian, Mei-Sheng Wu, Hong-Yuan Chen
Rok vydání:	2013
Předmět:	Silica nanoparticles Messenger RNA chemistry.chemical_compound Chemistry Electrode Electrochemiluminescence Liberation Breast cancer cells Molecular biology humanities DNA Intracellular
Zdroj:	Methods in Molecular Biology ISBN: 9781627035347
Popis:	Electrochemiluminescence (ECL) on bipolar electrode (BPE) is a sensitive, portable, and low-cost approach which has been employed to detect DNA and proteins. Here, we develop an ultrasensitive method for intracellular mRNA assay based on mRNA-mediated reporter DNA liberation and Ru(bpy)3(2+)-conjugated silica nanoparticles (RuSi@Ru(bpy)3(2+)) tag-based signal amplification.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::56075cca0ab290df145e503ab37ccc01 https://doi.org/10.1007/978-1-62703-535-4_15 Zobrazit plný text záznamu