Potential involvement of F0F1-ATP(synth)ase and reactive oxygen species in apoptosis induction by the antineoplastic agent erucylphosphohomocholine in glioblastoma cell lines
| Autor: | Wilfried Kugler, Yulia Shandalov, Sivan Zeno, Moshe Gavish, Karen Linnemannstöns, Julia von Alten, Leo Veenman, Max Lakomek |
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| Rok vydání: | 2010 |
| Předmět: |
Pharmacology
Mitochondrial ROS chemistry.chemical_classification 0303 health sciences Cancer Research Reactive oxygen species Oligomycin Myxothiazol biology Biochemistry (medical) Clinical Biochemistry Pharmaceutical Science Cell Biology Molecular biology 03 medical and health sciences chemistry.chemical_compound Mitochondrial membrane transport protein 0302 clinical medicine chemistry Mitochondrial permeability transition pore 030220 oncology & carcinogenesis Cardiolipin biology.protein Translocator protein 030304 developmental biology |
| Zdroj: | Apoptosis. 15:753-768 |
| ISSN: | 1573-675X 1360-8185 |
| DOI: | 10.1007/s10495-010-0460-5 |
| Popis: | Erucylphosphohomocholine (ErPC3, Erufosine™) was reported previously to induce apoptosis in otherwise highly apoptosis-resistant malignant glioma cell lines while sparing their non-tumorigenic counterparts. We also previously found that the mitochondrial 18 kDa Translocator Protein (TSPO) is required for apoptosis induction by ErPC3. These previous studies also suggested involvement of reactive oxygen species (ROS). In the present study we further investigated the potential involvement of ROS generation, the participation of the mitochondrial respiration chain, and the role of the mitochondrial FOF1-ATP(synth)ase in the pro-apoptotic effects of ErPC3 on U87MG and U118MG human glioblastoma cell lines. For this purpose, cells were treated with the ROS chelator butylated hydroxyanisole (BHA), the mitochondrial respiration chain inhibitors rotenone, antimycin A, myxothiazol, and the uncoupler CCCP. Also oligomycin and piceatannol were studied as inhibitors of the FO and F1 subunits of the mitochondrial FOF1-ATP(synth)ase, respectively. BHA was able to attenuate apoptosis induction by ErPC3, including mitochondrial ROS generation as determined with cardiolipin oxidation, as well as collapse of the mitochondrial membrane potential (Δψm). Similarly, we found that oligomycin attenuated apoptosis and collapse of the Δψm, normally induced by ErPC3, including the accompanying reductions in cellular ATP levels. Other inhibitors of the mitochondrial respiration chain, as well as piceatannol, did not show such effects. Consequently, our findings strongly point to a role for the FO subunit of the mitochondrial FOF1-ATP(synth)ase in ErPC3-induced apoptosis and dissipation of Δψm as well as ROS generation by ErPC3 and TSPO. |
| Databáze: | OpenAIRE |
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