A BODIPY-based 'OFF-ON' fluorescent probe for fast and selective detection of hypochlorite in living cells
| Autor: | Liyao Zheng, Dan Xu, Yan Huang, Jiaqin Hu, Kun Chen, Wancui Liu, Wen-Jing Shi, Fenggang Liu, Shu-Ting Chen |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Detection limit
chemistry.chemical_classification Chemistry Process Chemistry and Technology General Chemical Engineering chemistry.chemical_element Hypochlorite 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Photochemistry 01 natural sciences Fluorescence Photoinduced electron transfer 0104 chemical sciences Divalent chemistry.chemical_compound Fluorescence microscope BODIPY 0210 nano-technology Tellurium |
| Zdroj: | Dyes and Pigments. 170:107566 |
| ISSN: | 0143-7208 |
| DOI: | 10.1016/j.dyepig.2019.107566 |
| Popis: | A boron dipyrromethene (BODIPY)-based fluorescent probe bearing a tellurium atom was developed for fast and selective detection of hypochlorite (ClO−). Its molecular structure was firstly determined by X-ray diffraction analysis, indicating that the divalent tellurium formed a relatively small angle of 96.6(8)o and thus its electrons were easily oxidized. This probe could fast respond to ClO− and a strong green fluorescence appeared, attributing to oxidation of the tellurium and thereby inhibition of photoinduced electron transfer of the tellurium to the BODIPY. A linear fluorescence change was observed in the ClO−-concentration range of 0–8 μM with a detection limit of 0.12 μM. This probe was found to be applicable in a broad pH range (4–12). Confocal fluorescence microscopy imaging in RAW264.7 and HepG2 cells showed a remarkable green fluorescence enhancement with ClO−. This change could be also observed with phorbol myristate acetate-induced HClO. These results demonstrated that this probe could be an efficient fluorescent probe for ClO− detection in living cells. |
| Databáze: | OpenAIRE |
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