| Autor: |
L. Perlee, Ting Tang, Robert J. Hartzman, G.J. Coquillard, Jennifer Ng, Noriko Steiner, Carolyn Katovich Hurley |
| Rok vydání: |
2000 |
| Předmět: |
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| Zdroj: |
Tissue Antigens. 55:558-563 |
| ISSN: |
0001-2815 |
| DOI: |
10.1034/j.1399-0039.2000.550606.x |
| Popis: |
New HLA alleles are often identified initially from observing uncommon patterns found in low-resolution typing performed via polymerase chain reaction using sequence-specific oligonucleotide probes (PCR-SSOP). Recently, the HLA-DR oligotyping analysis of two Caucasian, one Caucasian/American Indian and two African American individuals resulted in the identification of three novel DRB3 alleles. Using DRB-specific primer sets commonly employed in amplification-based typing, all four individuals were originally characterized as DRB3 negative. Direct sequencing identified DRB3*0104 (variation at codon 8, T CG instead of T TG), and DRB3*0101202 (variation at intron (−13), G instead of C). One individual appeared to carry a DR52-associated DRB1 allele without an associated DRB3 allele. Lack of conservation at the junction of intron 1 and exon 2 of the DRB3 gene suggests that commonly used DRB-specific primer sets may need to be modified. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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