Protein differences among Populus deltoides internodal stem explants determined for shoot regeneration or callus growth

Autor:	Stephen G. Ernst, Gary D. Coleman
Rok vydání:	1991
Předmět:	biology fungi food and beverages Two dimensional page Plant Science General Medicine biology.organism_classification Basal shoot Tissue culture Salicaceae Callus Botany Shoot Genetics Agronomy and Crop Science Polyacrylamide gel electrophoresis Explant culture
Zdroj:	Plant Science. 75:83-92
ISSN:	0168-9452
DOI:	10.1016/0168-9452(91)90012-w
Popis:	Protein differences among internodal stem explants that were determined for either shoot regeneration or callus growth were studied by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and two dimensional PAGE. Three 32-kDa proteins with pI values of approximately 6.5–7.5 were detected only in callus inducing (CIM) and shoot inducing (SIM) treatments for which high levels of adventitious shoot regeneration were observed. A 35-kDa protein of approximately p I 7.0 was observed only in the CIM/SIM treatments which resulted in callus determined growth. These proteins may serve as markers to be used to characterize further the molecular events associated with the transition from competence for shoot regeneration to callus determined growth.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::55e20e51ac4e0cedf66205fff78cd5f9 https://doi.org/10.1016/0168-9452(91)90012-w Zobrazit plný text záznamu