| Autor: |
M. P. Betts, J.E. Beesley |
| Rok vydání: |
1987 |
| Předmět: |
|
| Zdroj: |
Micron and Microscopica Acta. 18:299-305 |
| ISSN: |
0739-6260 |
| DOI: |
10.1016/0739-6260(87)90030-x |
| Popis: |
Electron microscope examination of negatively stained preparations continues to be the method of choice for the diagnosis of virus particles although in some instances an immunological test is necessary. Colloidal gold immunocytochemical probes are becoming increasingly popular for electron microscopy and their suitability for the identification of virus particles is assessed. Virus particles were immunolabelled in situ on plastic/carbon coated electron microscope grids with specific antibody and colloidal gold probes. The labelling obtained was specific, definite and with very little background. The technique is very sensitive, very quick, and since a minimum of preparation is needed it appears to possess considerable potential for virus diagnosis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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